MODEL QUESTION PAPER
Time : 3 Hrs. Max. Marks : 30
- Make a temporary stained squash preparation of onion root tip. Identify, sketch and label any one stage of mitosis. Show your preparation to the examiner. (4)
Squash preparation 2 mark
Identification of stage 1mark
Labelled diagram 1 mark
In paper-1) Aim- Preparation of temporary stained squash preparation of onion root tip to study stage of mitosis by acetocarmine stain.
2) Requirements- Onion root tips, watch glass, 20% HCL, water, 1% acetocarmine stain, blade, needle, drawing brush, glass slides, coverslips, blotting papers, compound microscope.
3) Procedure – a) an onion root tip is taken in watch glass containing 20% HCL and heat it gently.
b) Keep it for about 2 minutes. HCL is removed by gently pouring water on the root tip.
C) In that watch glass add 2 drops of acetocarmine stain added and heated it gently.
d) Root tip is taken on slide and cutted the tip by blade.
e) The root tip is squashed by with the help of needle.
f) 1 drop of acetocarmine stain is added in that squash and squash is coverd by coverslip by avoiding air bubbles.
g) Excess stain is removed from slide by blotting paper
h) Slide is firstly observed under low power of microscope and then under high power of microscope to study the stage.
4) Observation- In slide of temporary squash prepared slide -----------stage of mitosis is observed.
5) characters of stage- 1)
2)
3)
4)
5) diagram-
OR
Dissect the given flower and display different whorls. Take a T.S. of ovary to show number of chambers. Sketch and label the diagram. (4)
display of floral whorls -2 mark
T. S. of ovary -1 mark
Labelled hidiagrm -1 mark
In paper- Aim- To dissect the given flower and display different whorls.
Requirements- Hibiscus flower, forceps, needles, slides, coverslip. new blade, dissecting microscope.
Procedure- 1. The given Hibiscus flower is dissected to separate the floral whorls like calyx, corolla, androecium and gynoecium.
2. all the floral whorls are mounted on the slide and observed.
3. T.S. of ovary is taken and counted the number of chambers in it.
diagram-
observation- the transverse section of ovary of Hibiscus shows 5 chambers hence it is pentalocular which shows axile placentation.
Que 2. Make a temporary preparation to show pollen germination. Draw neat and labelled diagram of the same. (3)
Preparation of slide of pollen germination -2 marks
Labelled diagram -1 mark
Aim- To study pollen grains germination on slide.
Requirements- Anthers of Hibiscus, sucrose solution containing 10 gm of sucrose, 20 gm. of potassium nitrate, 30 gm. of magnesium sulphate in 100 ml of water, slides, coverslips, compound microscope, etc.
Procedure-1. nutrient medium is prepared by dissolving 10 gm of sucrose,30 gm. of magnesium sulphate and 20 gm.potassium nitrate in 100 ml. of water.
2. few drops of nutrient medium are taken on a slide.
3. mature anthers are taken from the hibiscus flower and crushed on plain slide.
4. The pollen grains are collected with the help of brush and dusted on the slide having nutrient medium.
5. cover slip is placed on the slide
6. slide is observed under compound microscope after 5 minutes up to 30 minutes.
Diagram-
Observation- 1. germinated pollen grains are seen on the slide.
2. A pollen grain has a distinct exine with germ pore.
3. below the exine , intine is present .
4. exine is thick , irregular and rough in nature.
5. intine is thin , uniform and smooth in nature.
6. intine forms pollen tube which comes out through the germ pore.
OR
L
Comment upon the physiological experiment set up. (3)
aim 1 mark
observation and conclusion 2 marks
On paper-
Aim- to separate plant pigments by paper chromatography
requirements- Spinach leaves, mortar and pistal, sand, muslin cloth, chromatography chamber with lid, whatman chromatography paper no 1, thread, capillary, funnel, beaker, measuring cylinder, acetone,petroleum ether, CaCo3 powder.
observation- on the strip we can observe the separation of photosynthetic photosynthetic pigments in the form of bands of different colours in a particular sequence from the loaded point up to the top of chromatograph.the colour bands are yellowish green, bluish green , yellow and orange.
conclusion- the spinach leaf contains photosynthetic pigments like chlorophyll-chlorophyll-a, xanthophyll and carotene.
OR
Calculate population density/frequency of any one plant species by quadrat method. (3)
observation table and calculation 2 marks
result 1 mark
on paper- Aim- to study plant population density and frequency by quadrat method.
Requirements- field having plants of different species, meter scale, Paper, Pencil, sting in the selected field.
procedure- 1. area of land is selected having various species of plants.
2. with the help of nails , sting and meter scale four square quadrat of 1m × 1m are ploted and named as A,B,C,D.
3. identified the different species of plants in these quadrates
4. number of plant species in these quadrates is noted in observation table
observation table-
calculation-
total number of plants in the species
Density =
Total number of quadrats studied
Total number of quadrats studied
Total number of quadrats in which species occurred
Frequency = 
Total number of quadrats studied
Result- 1. density of parthenium plant species is---------
% frequency of parthenium plant species is-----------
2. density of cynodon plant species is---------
% frequency of cynodon plant species is-----------
3. density of oxalis plant species is---------
% frequency of oxalis plant species is-----------
Que-3 Detect the presence of and constituents from the given sample of urine.
Make a note of their clinical significance. (4)
a) urea and albumin
b) sugar and albumin
Performance and result for each component 1+1 marks
clinical significance of each component 1 mark
aim- detection of -----------and ----- from given sample of urine.
Requirments-
observation table-
TEST FOR ---------------
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TEST
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OBSERVATION
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INFERENCE
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TEST FOR ----------
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TEST
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OBSERVATION
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INFERENCE
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Result- In given sample of urine ----------and------------are present.
clinical significance-
urea- In healthy human being , urea is present about 60-150 mg/1lit of blood.
urea is broken down by urease enzyme to librate ammonia and carbon dioxide.
albumin- albumins are the proteins. these proteins having high molecular weight substances which are not filtered during ultrafiltration. when there is infection, inflamation or disorder in the malpighian bodies , proteins as well as blood cells appear in urine .
Sugar-due to the deficiency of insulin glucose is not converted into glycogen.the presence of glucose in urine is called as glucoseurea,and disorder is called as diabetes millitus.
Que-4. Perform the suitable test to compare the given water samples for their pH and clarity. Make a note of their pH and clarity. (3)
1/2 mark for detecting ph of each water sample
1 mark for detecting clearity of each sample.
Aim- to detect the pH and clarity of given water samples A and B.
Requirements- water samples pH papers, bottles with caps.
observation table-
observation-
1. the given water sample A having 7 pH which is referred as neutral , while water sample B having pH 6.2 referred as Acidic.
2. the given water sample A is clear and water sample B is turbid due to presence of suspended particles which reduces clarity of water.
conclusion- water sample A is suitable for drinking while water sample B is not suitable for drinking without treatment.
OR
Que-4. Perform the suitable test to compare the given soil samples for their pH and type. Make a note of their pH and soil type. (3)
1/2 mark for detecting pH of each sample
1 mark for detecting type of each sample
Aim-To study the pH and type study of the given soil samples.
requirmatns- Measuring cylinders, water , pH papers, glass rods.
observation table-
Conclusion- 1. soil sample A contains ----% of sand,----% of slit and ----% of clay hence the soil type is ------------ having pH----------
2. soil sample B contains ----% of sand, ----% of slit and ----% of clay hence the soil type is ------------ having pH----------
OR
Que-4 Pefrform the experiment to compare the given samples of leaves for the presence of particulate matter. Make a note of the same. (3)
1 1/2 mark each for reporting the particulate matter
Aim-to study the presence of particulate matter in air collected from widely different sites
requirements-sides, ear buds, microscopes, unwashed leaves of road side plants . washed leaves.
observation
Unwashed leaves shows many air pollutants such as dust particles, carbon particles, pollen grains, spores.
Washed leaves does not show anyparticulate matter on the leaf .
Que-5. Identify the spots 'A' to 'F' as per the instructions. (6)
A. given material is for the Anemophily in Maize.
Adaptations- 1. Large number of pollen grains are produced as there is possibility of wastage of pollen grains due to wind.
- Pollen grains are dry, light in weight and smooth walled.
- Flowers are minute and not attractive.
- Nectar and fragrance are absent.
- Calyx and corolla are replaced by perianth.
- Male flowers are at the terminal position in the inflorescence .
- Female flowers are at axillary position present at lower position for catching pollen grains.
- Stigma is feathery or brush like for catching pollen grains.
Or
- Given material is for Entomophily in salvia.
1.flowers are attractive and having bright colour.
2.flower having nectar and nectar producing glands situated in such a way that the insects body parts touches the anther and stigma during collecting thenectar.
3.the corolla is bilabite or bilipped.
4.the filament of stigma is elongated, having two lobes – upper fertile lobe and lower sterile lobe.
5.sterile lobe is useful for the lagging of insect during collecting nectar.
6.when insect lag on the lower lobe of stigma upper lobe comes in the contact with insect body by which pollen grains attaches to the body parts of insects.
B.given spot B is bagging step for cross pollination.
1. the emasculated flowers are covered with perforated polythene bags. They are allowed aeration but prevent pollination by foreign pollens.
2. the flowers of male parent are also covered by perforated polythene bags to avoid mixing with foreign pollens.
Or
- given slide is of meiosis stage
the given spot B contains slide for the -----------stage of meiosis
characters-1.
2.
3.
4.
5.
C. The given spot c is the specimen for the study of xerophytic modifications in Opuntia.
Characters- 1.It is succulent droughtresistant xerophyte.
2. The stem is flattened green called phylloclade, which carry the function of photosynthesis.
3. the stem is fleshy due to presence of mucilage which retains water.
4. phylloclade shows several leaves which are modified into spines to reduce transpiration.
5. bristles are present to prevent grazing by animals.
Or
- the given spot C is the specimen for the study of aquatic modifications in Hydrilla.
Characters- 1. It is submerged hydrophyte which grows initially under water.
2. It is attached to the substratum by adventitious roots infreshwater ponds.
3. The stem is slender and soft. It is without mechanical tissue, hence limps when taken out of water.
4. leaves are very thin, membranous and are arranged in whorls . they lacks cuticle and stomata.
5. the entire plant is covered by mucilage that protect it from rotting effect of water.
D. the given spot D is T.S. of testis
1.the mammalian testis is covered by a thick fibrous layer called tunica albuginea.
2.the testis contain large number of seminiferous tubules
3.seminiferous tubules are lined by germinal epithelial cells and sertoli cells.
4.germinal epithelial cells produces sperms by the process of spermatogenesis
5.sertoli cells provides nourishment to the developing sperms.
6. in between the seminiferous tubules leydig cells are present producing testosterone
Or
- The given spot D is T.S. of ovary.
1.The mammalian ovary is externally lined by germinal epithelium, followed by thick layer of fibrous layer, tunica albuginea.
2.ovary consist of outer part cortex while inner part medulla.
3.cortex contains large number of developing stages of oocytes
4.medulla contains fibrous connective tissue containing blood vessels, nerves .
5.cortrex also contains large yellow body cell called as corpus luteum which secrete female sex hormone progesterone.
E. the given spot E is the pedigree chart for color blindness.
1.
2.
3.
4.
Or
- The given spot E is the pedigree chart for blood group.
1.
2.
3.
4.
5.
F. The given spot F is the pathogen Ascaris lumbricoides.
1. A ascaris is an endoparasite of the small intestine of human being and is more common in children's.
2. The animal shows sexual dimorphism. The female is larger while male is smoller.
3. the posterior end of male is curved ventrally.
4. In male pineal setae are present at the posterior side .
5.the infection of ascaris causes abdominal discomfort like colic pains, impaired digestion, diarrhea, vomiting .
6. In children mental efficiency is affected and body growth is retarded.
Or
- The given spot F is Rohu showing aquatic adaptation.
1.The body is streamlined to minimize resistance of water.
2.Gills are present for respiration .
3.paired and unpaired fins are present for swimming.
4.body is covered by scales.
5.lateral line system helps in sensing the surrounding.
6. Air bladder is present in bony fishes for buoyancy to the body.
Standard Xll (Upgraded)
Practical Examination in Biology Question Paper
Batch – I / II /III/IV
Date- Time-
Time: 3 Hours Max. Marks: 30
Q. 1 Make a temporary stained squash preparation of onion root tip. Identify, sketch and label any one stage of mitosis. Show your preparation to the examiner. 4
Or
Dissect the given flower and display different whorls. Take a T.S. of ovary to show number of chambers. Sketch and label the diagram. 4
Q.2 Make a temporary preparation to show pollen germination. Draw neat and labelled diagram of the same. 3
Or
Comment upon the physiological experiment set up. 3
………………………………………………………………………
Or
Calculate population density/ frequency of any one plant species by quadrat method. 3
Q.3 Detect the presence of…………………and………………..constituents from
the given sample of urine. Make a note of their clinical significance. 4
Q.4 Perform the suitable test to compare the given water samples for their pH and clarity. Make a note of their pH and clarity. 3
Or
Perform the suitable test to compare the given soil samples for their pH and type. Make a note of their pH and soil type. 3
or
Perform the experiment to compare the given samples of leaves for the presence of particulate matter. Make a note of the same. 3
Q.5 Identify the spots 'A' to 'F 'as per the instructions. 6
Q.6 Project report. 5
Q.7 Viva voce based on first four questions. 2
Q.8 Journal/ Record book 3
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